HPLC-MS Analysis and Antioxidant Activity Assessment of Moringa oleifera Leaf Extrac t: Identification of Bioactive Compounds and Therapeutic Potential
DOI:
https://doi.org/10.32628/IJSRST2511145Keywords:
Moringa Oleifera, HPLC-MS, Antioxidant Activity, Bioactive Compounds, Phytochemical ScreeningAbstract
This study presents a comprehensive HPLC-MS analysis and antioxidant activity evaluation of Moringa oleifera leaf extract, a medicinal plant renowned for its nutritional and therapeutic properties. Fresh Moringa leaves were collected, processed through shade drying, and extracted using methanol through maceration followed by liquid-liquid extraction. High-performance liquid chromatography coupled with mass spectrometry (HPLC-MS) was employed to identify and quantify bioactive compounds. The analysis revealed the presence of 15 major compounds including quercetin, kaempferol, chlorogenic acid, rutin, caffeic acid, ferulic acid, and gallic acid. Phytochemical screening confirmed the presence of flavonoids, phenolic compounds, alkaloids, and saponins in varying concentrations across different extraction solvents. Antioxidant activity assessment using DPPH and ABTS assays demonstrated significant free radical scavenging capacity with IC₅₀ values of 89.5 μg/mL and 76.3 μg/mL respectively. The methanol extract showed superior antioxidant activity compared to other solvent extracts, correlating with higher flavonoid and phenolic content. These findings support the traditional use of Moringa oleifera in folk medicine and provide scientific basis for its development into pharmaceutical formulations and functional foods.
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References
A. Fahey, "Moringa oleifera: A review of the medical evidence for its nutritional, therapeutic properties," Trees for Life Journal, vol. 1, no. 5, pp. 1-15, 2005.
S. Gopalakrishnan et al., "Moringa oleifera: A review on nutritive importance and its medicinal application," Food Science and Human Wellness, vol. 5, no. 2, pp. 49-56, 2016. DOI: https://doi.org/10.1016/j.fshw.2016.04.001
M. Mbikay, "Therapeutic potential of Moringa oleifera leaves in chronic hyperglycemia," Frontiers in Pharmacology, vol. 3, article 24, 2012. DOI: https://doi.org/10.3389/fphar.2012.00024
N. Pandey et al., "Phytochemical and pharmacological properties of Moringa oleifera: A review," International Journal of Pharmaceutical Sciences, vol. 5, no. 2, pp. 1-11, 2011.
C. Anwar et al., "Effect of drying method on bioactive compounds of leaves of drumstick tree," LWT-Food Science and Technology, vol. 40, no. 8, pp. 1-8, 2007.
K. Sreelatha and R. Padma, "Antioxidant activity and total phenolic content of Moringa oleifera leaves," International Journal of Food Properties, vol. 12, no. 2, pp. 405-412, 2009.
B. Halliwell and J. Gutteridge, "Free radicals in biology and medicine," 4th ed. Oxford: Oxford University Press, 2007.
A. Coppin et al., "Identification and quantification of flavonoids and phenolic acids in moringa oleifera leaves by HPLC-DAD-ESI-MSn," Food Chemistry, vol. 145, pp. 1-8, 2014.
P. Siddhuraju and K. Becker, "Antioxidant properties of various solvent extracts of total phenolic constituents from moringa oleifera leaves," Journal of Agricultural and Food Chemistry, vol. 51, pp. 2144-2155, 2003. DOI: https://doi.org/10.1021/jf020444+
W. Brand-Williams et al., "Use of free radical method to evaluate antioxidant activity," LWT-Food Science and Technology, vol. 28, pp. 25-30, 1995. DOI: https://doi.org/10.1016/S0023-6438(95)80008-5
R. Re et al., "Antioxidant activity applying improved ABTS radical cation decolorization assay," Free Radical Biology and Medicine, vol. 26, pp. 1231-1237, 1999. DOI: https://doi.org/10.1016/S0891-5849(98)00315-3
C. Rice-Evans et al., "Structure-antioxidant activity relationships of flavonoids," Free Radical Biology and Medicine, vol. 20, no. 7, pp. 933-956, 1996. DOI: https://doi.org/10.1016/0891-5849(95)02227-9
P. Cos et al., "Structure-activity relationship and classification of flavonoids as inhibitors of xanthine oxidase," Journal of Natural Products, vol. 61, pp. 71-76, 1998. DOI: https://doi.org/10.1021/np970237h
A. Vongsak et al., "Maximizing total phenolics content and antioxidant activity in moringa oleifera leaf extracts," International Journal of Food Science, vol. 2013, article 961315, 2013.
M. Atawodi et al., "Evaluation of the polyphenol content and antioxidant properties of methanol extracts of the leaves of moringa oleifera," Molecules, vol. 15, no. 12, pp. 9235-9243, 2010.
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