Influence of Azolla filiculoides on Dairy Cow Milk Production and Improvements in Macro Nutrients and Milk Quality Indicators
DOI:
https://doi.org/10.32628/IJSRST2613154Keywords:
Azolla filiculoides, Nutritional quality of milk, Milk composition analysis, Dairy cattle nutrition, Sustainable dairy farmingAbstract
The dairy industry in India plays a pivotal role in rural development, employment, and nutritional security, making the quality of milk produced a key factor for both consumer health and economic viability. Enhancing milk quality, particularly in terms of fat, protein, and casein content, is essential for the production of high-value dairy products. Azolla filiculoides, a nutrient-rich and sustainable feed supplement, has shown potential to significantly improve these milk quality parameters. In a study involving 20 lactating HF crossbreed cows, Azolla supplementation led to notable increases in milk yield, with improvements ranging from 13.70% to 30.89%. Milk fat content ranged from 2.49% to 4.98%, protein content increased from 2.63%-3.24% to 3.16%-3.98%, and casein levels improved from 2.01%-2.3% to 2.66%-3.04%. Minimal changes were observed in lactose and galactose levels, indicating that while Azolla enhances fat and protein content, it does not significantly alter milk sugars. These results highlight A. filiculoidesas an effective, sustainable feed supplement that enhances milk quality, supporting the dairy industry's objectives of improving nutritional outcomes and economic sustainability.
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Abdulwahab, I. A., Yasin, S. R., Jasim, S. S., Abdullah, S. R. S., Almansoory, A. F., & Ismail, N. I. 2022. Removal of copper by A. filiculoides and Lemna minor: Phytoremediation potential, adsorption kinetics, and isotherms. Heliyon, 8: e11456. DOI: https://doi.org/10.1016/j.heliyon.2022.e11456
Acharya, P., Singh, Y., Sharma, A., Pattnaik, N., & Prakash, A. 2023. Azolla: An alternative feed for sustainable livestock production. International Journal of Veterinary Sciences and Animal Husbandry, 8: 10-16. DOI: https://doi.org/10.22271/veterinary.2023.v8.i3Sa.595
Ahmad, M. I., Senusi, W., Binhweel, F., &AlSaadi, S. A. 2023. Optimization of base catalytic transesterification toward maximum biodiesel yield from A. filiculoides macroalgae feedstock. Industrial Crops and Product, 197: 116590. DOI: https://doi.org/10.1016/j.indcrop.2023.116590
Alebachew, S., Zerihun, T., Nigussie, B., & Amsalu, F. 2024. Azolla as a beneficial macrophyte for livestock feed: A review. Cogent Food & Agriculture. 10: 2367804. DOI: https://doi.org/10.1080/23311932.2024.2367804
Anitha, K. C., Rajeshwari, Y. B., Prasanna, S. B., & Shilpa, J. 2016. Nutritive evaluation of Azolla as livestock feed. Journal of Experimental Biology and Agricultural Sciences, 4: 670-674. DOI: https://doi.org/10.18006/2016.4(Issue6).670.674
Azevedo, R. A., Lage, C. F. A., Silper, B. F., DinizNeto, H. C., Quigley, J. D., & Coelho, S. G. 2023. Invited review: Total solids concentration in milk or milk replacer for dairy calves. Journal of Dairy Science, 106: 7341-7351. DOI: https://doi.org/10.3168/jds.2023-23372
Balasubramanian, G. 2024. Growth and development of dairy industry in India. European Economic Letters, 14: 451–462.
Bhat, M. Y., Dar, T. A., & Singh, L. R. 2016. Casein proteins: Structural and functional aspects. InTech. https://doi.org/10.5772/64187. DOI: https://doi.org/10.5772/64187
Bhatt, N., Singh, N. P., Singh, A., Kandpal, D., Chaudhary, P., &Patoliya, P. 2020. Azolla – A potent unconventional feed and its effect on feeding various livestock species: A review. Journal of Entomology and Zoology Studies, 8: 1693-1698.
Biswas, S., Mandal, A., Chakraborty, M., & Biswas, K. 2021. Determination of fat, SNF and protein content in cow milk from the voltage output of ‘MilkTester’. I2MTC 2021 - IEEE International Instrumentation and Measurement Technology Conference Proceedings. pp. 1-5. DOI: https://doi.org/10.1109/I2MTC50364.2021.9459873
Celik, B. 2022. Effect of citric acid on biofilm formed by P. fluorescens strains isolated from raw milk samples offered for consumption. International Journal of Pathogen Research, 9: 47-55. DOI: https://doi.org/10.9734/ijpr/2022/v9i330229
Chaudhary, R., Rai, S., Sailo, L., Farooq, U. B., Singh, A., Naha, B. C., & Kumar, A. 2017. Genetic and non-genetic factors influencing fatty acid composition of dairy milk: A review. Indian Journal of Animal Nutrition, 34: 1. DOI: https://doi.org/10.5958/2231-6744.2017.00001.9
Chen, J., Huang, M., Cao, F., Pardha-Saradhi, P., & Zou, Y. 2017. Urea application promotes amino acid metabolism and membrane lipid peroxidation in Azolla. PLOS ONE, 12: e0185230. DOI: https://doi.org/10.1371/journal.pone.0185230
Christy, E., Wickramasinghe, I., Kapilan, R., &Wijesekara, I. 2024. Enhancing bioethanol production from A. filiculoides through optimization of pretreatment and culture conditions with Saccharomyces cerevisiae. Journal of Agricultural Sciences, 19: 171-187. DOI: https://doi.org/10.4038/jas.v19i1.9906
Chu, C. Y., & Wang, C. C. 2013. Toxicity of melamine: The public health concern. Journal of Environmental Science and Health, Part C: Environmental Carcinogenesis & Ecotoxicology Reviews, 31: 342–386. DOI: https://doi.org/10.1080/10590501.2013.844758
Dubljević, R., Marković, B., Radonjić, D., Stešević, D., &Marković, M. 2020. Influence of changes in botanical diversity and quality of wet grasslands through phenological phases on cow milk fatty acid composition. Sustainability, 12: 6320. DOI: https://doi.org/10.3390/su12166320
El-Naggar, S., & El-Mesery, H. S. 2022. Azollapinnata as unconventional feeds for ruminant feeding. Bulletin of the National Research Centre, 46: 66. DOI: https://doi.org/10.1186/s42269-022-00752-w
Fedorova, A., Kozlova, O., Slavyanskiy, A., Velichkovich, N., & Le, V. M. 2020. Research and development of maltodextrin production technology for the dairy industry. Food Processing: Techniques and Technology, 50: 616-629. 10.21603/2074-9414-2020-4-616-629 DOI: https://doi.org/10.21603/2074-9414-2020-4-616-629
Gao, X., & Oba, M. 2016. Effect of increasing dietary nonfiber carbohydrate with starch, sucrose, or lactose on rumen fermentation and productivity of lactating dairy cows. Journal of Dairy Science, 99: 291-300. DOI: https://doi.org/10.3168/jds.2015-9871
Gowda, N. K. S., Manegar, A., Shiv, S., Verma, N. C., Valleesha, G., Maya, G., Pal, D. T., & Suresh, K. P. 2015. Azolla (Azollapinnata) as a green feed supplement for dairy cattle: An on-farm study. Animal Nutrition and Feed Technology, 15: 283. DOI: https://doi.org/10.5958/0974-181X.2015.00031.1
Guerville, M., &Ligneul, A. 2024. "Lactose: A Sugar Unlike Any Other." Cahiers de Nutrition et de Diététique, 59: 102-112. 59: 102-112. DOI: https://doi.org/10.1016/j.cnd.2023.12.004
Guliński, P. 2024. The effect of different content of protein and urea in milk, as biomarkers of energy-protein balance of food rations, on the level of selected milk performance characteristics of Polish Holstein-Friesian cows. Acta ScientiarumPolonorum, 34: 5-23.
Hanuš, O., Vegricht, J., Frelich, J., Macek, A., Bjelka, M., Louda, F., &Janů, L. 2018. Analysis of raw cow milk quality according to free fatty acid contents in the Czech Republic. Czech Journal of Animal Science, 53: 17-30. DOI: https://doi.org/10.17221/2717-CJAS
Hassen, A., Ahmed, R., Alam, M., Chavula, P., Shek, S., &Dawid, A. 2022. The effect of feed supplementation on cow milk productivity and quality: A brief study. International Journal of Agriculture and Veterinary Sciences, 10: 13-25. DOI: https://doi.org/10.34104/ijavs.022.013025
Huppertz, T. 2022. Lactose in milk: Properties, nutritional characteristics, and role in dairy products. In: Huppertz, T., & Vasiljevic, T. (Eds.), Understanding and improving the functional and nutritional properties of milk. BurleighDodds Science Publishing, pp. 361-380. DOI: https://doi.org/10.19103/AS.2022.0099.10
Kantwa, S. C., Kumar, U., & Patel, S. J. 2022. Azolla as a green fodder feed supplement of cross-breed cows in district-Sehore (M.P.). Bhartiya Krishi AnusandhanPatrika, 36: 280-284. DOI: https://doi.org/10.18805/BKAP378
Kerketta, S., Sarangdevot, S. S., Naruka, P. S., Verma, S., Pachauri, C. P., Singh, A. K., Singh, J. P., &Bhadauria, S. S. 2020. Effect of Azolla as feed supplement on milk production of lactating buffaloes at Neemuch district Madhya Pradesh. Indian Journal of Dairy Science, 73: 4. DOI: https://doi.org/10.33785/IJDS.2020.v73i04.016
Kolhal, M. T. 2022. Indian dairy industry. International Journal of Health Sciences (IJHS), 6: 10880–10885. DOI: https://doi.org/10.53730/ijhs.v6nS2.7910
Kondrotienė, K., Zavistanavičiutė, P., Aksomaitienė, J., Novoslavskij, A., &Malakauskas, M. 2023. Lactococcus lactis in dairy fermentation—Health-promoting and probiotic properties. Fermentation, 10: 16. DOI: https://doi.org/10.3390/fermentation10010016
Kösesakal, T., &Seyhan, M. 2022. Naphthalene stress responses of the aquatic fern A. filiculoides Lam. and evaluation of phytoremediation potential. Polycyclic Aromatic Compounds, 43: 6819-6836. DOI: https://doi.org/10.1080/10406638.2022.2126505
Kour, M., Khan, N., Singh, R., Mahajan, V., Amrutkar, S. A., & Kumar, D. 2020. Effect of Azolla (Azollapinnata) supplementation on milk yield, composition, and economics in crossbred HF cows. International Journal of Current Microbiology and Applied Sciences, 9: 2661-2666. DOI: https://doi.org/10.20546/ijcmas.2020.910.320
Kovalenko, V., Kovalenko, N., Gamayunova, V., Butenko, A. O., Kabanets, V., Salatenko, I., Kandyba, N., &Vandyk, M. 2024. Ecological and technological evaluation of the nutrition of perennial legumes and their effectiveness for animals. Journal of Ecological Engineering, 25: 294-304. DOI: https://doi.org/10.12911/22998993/185219
Kumar, U., Reager, M. L., &Mitharwal, B. S. 2020. Cultivation of Azolla (Azollapinnata) and its use as cattle feed supplement. International Journal of Current Microbiology and Applied Sciences, 9: 1174-1178. DOI: https://doi.org/10.20546/ijcmas.2020.905.129
Li, M. M., Huang, X., Campos, L. M., Prestegaard-Wilson, J. M., Pilonero, T., Budde, A., & Hanigan, M. D. 2023. Effects of dietary starch and ruminally undegraded protein on glucogenic precursors in lactating dairy cows. Animal,17( 5): 100893. DOI: https://doi.org/10.1016/j.animal.2023.100893
Mahajan, V., & Singh, J. 2022. Lives, livelihoods and environment: The challenge of sustainable development goals in India. In: Antoniades, A., Antonarakis, A. S., &Kempf, I. (Eds.), Financial crises, poverty and environmental sustainability: Challenges in the context of the SDGs and Covid-19 recovery. Springer, Cham, pp. 169-185. DOI: https://doi.org/10.1007/978-3-030-87417-9_7
Mansour, H. A., Almatarneh, A. A., Abu-Saleh, A. A., &Elayan, I. A. 2017. Mechanistic and spectral investigation on the deamination of ammeline and ammelide. Computational and Theoretical Chemistry, 1117: 92-99. DOI: https://doi.org/10.1016/j.comptc.2017.08.006
Mathur, G. N., Sharma, R., & Choudhary, P. 2013. Use of Azolla (Azollapinnata) as cattle feed supplement. Journal of Krishi Vigyan, 2: 73-75.
Miciński, J., Zwierzchowski, G., Kowalski, I. M., &Szarek, J. 2012. Health-promoting properties of selected milk components. Journal of Elementology, 18: 165-186. DOI: https://doi.org/10.5601/jelem.2013.18.1.14
Mudgal, V. D., Jaiswal, P., Abraham, G., Kaushik, G. K., & Dahiya, S. S. 2022. Aquatic fern Azolla affecting milk production in buffaloes. Buffalo Bulletin, 41: 579-589. DOI: https://doi.org/10.56825/bufbu.2022.4143543
Nasir, N. A. N. M., Kamaruddin, S. B. A., Zakarya, I. A., & Islam, A. K. M. A. 2022. Sustainable alternative animal feeds: Recent advances and future perspective of using Azolla as animal feed in livestock, poultry, and fish nutrition. Sustainable Chemistry and Pharmacy, 25: 100581. DOI: https://doi.org/10.1016/j.scp.2021.100581
Nayana, M. S., Deepak, N., Nisha, M., Shravani, M. S., & Manjunath, H. R. 2023. A review on milk quality detection and analysis. International Journal of Advanced Research in Science, Communication and Technology (IJARSCT), 3: https://doi.org/10.48175/IJARSCT-7838. DOI: https://doi.org/10.48175/IJARSCT-7838
Onaciu, G., Jurco, E., Jurco, S. G., Maciuc, V., &Ognean, L. 2019. Influence of varying ranges of milk urea nitrogen on chemical, hygienic, and physical quality traits of cow milk. Romanian Biotechnological Letters, 24: 866-873. DOI: https://doi.org/10.25083/rbl/24.5/866.873
Oshima, M., & Fuse, H. 1981. Citric acid concentration in subclinical mastitic milk. Journal of Dairy Research, 40: 32-36.
Pandey, S., Mishra, S., Jha, A. K., & Pandey, H. O. 2024. An overview of recent dairy schemes revolutionizing the socio-economic landscape of India. International Journal of Agriculture Extension and Social Development, 7: 229-235. DOI: https://doi.org/10.33545/26180723.2024.v7.i2d.332
Quiroz, D. A. A. 2022. Lactic acid bacteria in raw-milk cheeses: From starter cultures to probiotic functions. Foods, 11: 2276. DOI: https://doi.org/10.3390/foods11152276
Ramani, A., Deshmukh, R., Seth, R., Gandhi, K., & Sharma, R. 2023. Development of dairy industry: An identity of India’s self-reliance - A review. Bhartiya Krishi AnusandhanPatrika, 38: 203-209. DOI: https://doi.org/10.18805/BKAP625
Rao, D. 2016. Safety of milk processing and distribution chain in India. In: Prakash, V., Martín-Belloso, O., Keener, L., Astley, S., Braun, S., McMahon, H., &Lelieveld, H. (Eds.), Regulating safety of traditional and ethnic foods. Academic Press, pp. 169-185. DOI: https://doi.org/10.1016/B978-0-12-800605-4.00009-8
Ravelo, A., Vyas, D., Ferraretto, L. F., &Faciola, A. P. 2022. Effects of sucrose and lactose as partial replacements for corn in lactating dairy cow diets: A review. Translational Animal Science, 6: 1-8. DOI: https://doi.org/10.1093/tas/txac044
Rawat, N., Kumari, K., Singh, F. V., &Gilhare, V. R. 2015. Effect of Azolla-supplemented feeding on milk production of cattle and production performance of broilers. Applied Biological Research, 17: 214. DOI: https://doi.org/10.5958/0974-4517.2015.00031.2
Salman, M., El-Sayed, S. A., Al-Amoudi, M. S., Alghamdi, L. S., &Bazaid, S. A. 2012. Identification and determination of melamine in milk by high-performance liquid chromatography-UV detector. Scholars Research Library, 4: 737-748.
Sasaoka, N., Imamura, H., &Kakizuka, A. 2018. A trace amount of galactose, a major component of milk sugar, allows maturation of glycoproteins during sugar starvation. iScience,10: 211-221. DOI: https://doi.org/10.1016/j.isci.2018.11.035
Sundararaman, S., Karthikeyan, M., Aravind Kumar, J., et al. 2024. Facile synthesis of iron nanoparticles from Camellia sinensis leaves catalyzed for biodiesel synthesis from A. filiculoides. Scientific Reports, 14: 12818. DOI: https://doi.org/10.1038/s41598-024-61113-3
Szilagyi, A. 2019. Digestion, absorption, metabolism, and physiological effects of lactose. In: Paques, M., & Lindner, C. (Eds.), Lactose. Academic Press, pp. 49-111. DOI: https://doi.org/10.1016/B978-0-12-811720-0.00002-7
Tirfie, F. W. 2023. A review of genetic and non-genetic parameter estimates for milk composition of cattle. Animal and Veterinary Sciences, 11: 64-70.
Vyawahare, A. S., Nawale, P., Kumar, S., Papinwar, K., Dhinalkumar, H. P., & Rao, K. J. 2011. Psychorheological study on viscosity of milk. Journal of Dairying, Foods and Home Sciences, 30: 1.
Yusa, K., Ando, K., & Onodera, Y. 1969. Citric acid content of cow's milk. Nihon ChikusanGakkaiho, 40: 32-36. DOI: https://doi.org/10.2508/chikusan.40.32
Zazouli, M. A., Ala, A., Asghari, S., &Babanezhad, E. 2023. Evaluation of A. filiculoides potential in pyrene and phenanthrene accumulation and phytoremediation in contaminated waters. International Journal of Phytoremediation, 26: 608-617. DOI: https://doi.org/10.1080/15226514.2023.2257314
Zhao, G. 2018. Improving feed protein utilization rate in cattle through nutritional approaches. Current Protein & Peptide Science, 20: 164-171. DOI: https://doi.org/10.2174/1389203719666180514153236
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